Rumlow attempts to kill Rogers by exploding himself but Maximoff accidentally sends him spiralling into a building, causing many casualties. Steve Rogers, Natasha Romanoff, Sam Wilson and Wanda Maximoff stop Brock Rumlow from stealing a biological weapon in Lagos. In the present day 2016, it has been a year since Ultron's destruction. In 1991, James "Bucky" Barnes is sent from a HYDRA base in Siberia to attack a car carrying vials of the Super Soldier Serum. Helicarriers (Appears on a computer screen, TV, or hologram only)
Spider-Man's Utility Belt (First appearance). Iron Man Armor MK XLVI (First appearance). Tony Stark's Sunglasses (First appearance). 
Spider-Man's Spider-Signal (First appearance).Spider-Man's Web-Shooters (First appearance).War Machine Armor MK III (First appearance).Super-Soldier Serum (Only in flashback).
Winter Soldier's Bionic Arm (Destruction). Memory Supression Device (Only in flashback). Institute for Infectious Diseases Building. Triskelion (Appears on a computer screen, TV, or hologram only). Potomac River (Appears on a computer screen, TV, or hologram only). (Appears on a computer screen, TV, or hologram only) Avengers Tower (Appears on a computer screen, TV, or hologram only). Manhattan (Appears on a computer screen, TV, or hologram only). United States of America (Main story and flashback). North America (Main story and flashback). Joint Counter Terrorist Centre Building. Novi Grad (Appears on a computer screen, TV, or hologram only). Sokovia (Appears on a computer screen, TV, or hologram only). Hydra Siberian Facility (Main story and flashback). Earth-199999 (Main story and flashback). Leviathans (Appears on a computer screen, TV, or hologram only). Chitauri (Appears on a computer screen, TV, or hologram only). EKO Scorpion (Appears on a computer screen, TV, or hologram only). Peggy Carter (Behind the scenes) (Death). 
Hulk (Bruce Banner) (Appears on a computer screen, TV, or hologram only). Charlie Spencer (Photo) (Only appearance) (Deceased). Howard Stark (Only in flashback) (Death). Maria Stark (Only in flashback) (Death). Winter Soldiers (Appear in flashback and main story) (Only appearance die). Vasily Karpov (Main story and flashback) (Only appearance dies) Helmut Zemo (First appearance) (Impersonates Dr. Crossbones' mercenaries (Only appearance). Spider-Man (Peter Parker) (First appearance). Black Panther (Prince T'Challa) (First appearance). Joint Counter Terrorist Centre (First appearance). Black Widow (Natasha Romanoff) (Leaves team). Winter Soldier (Bucky Barnes) (Main story and flashback). Iron Man (Tony Stark) (Rejoins the Avengers). Captain America (Steve Rogers) (Leaves the Avengers).
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